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mouse anti ap2a  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti ap2a
    Mouse Anti Ap2a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ap2a/anti-AP-2+alpha/pm41571667-247-58-60
    Average 96 stars, based on 103 article reviews
    mouse anti ap2a - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    In Situ Hybridization:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).

    CRISPR:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).

    Recombinant:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).

    Purification:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).

    Magnetic Beads:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).

    Electroporation:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).

    Extraction:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).

    Software:

    Article Title: A cell atlas of the chick retina based on single-cell transcriptomics
    Article Snippet: Antibody , Anti-AP2A (Mouse monoclonal) , Developmental Studies Hybridoma Bank , Clone name: 3B5 , IF(1/100).



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    mouse anti ap2a  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank mouse anti ap2a
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    ap2a  (Cell Signaling Technology Inc)
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    Fig. 3 HB-EGF and EGFR control miR-221 expression that targets <t>AP2a</t> expression. A Fold change in AP2a expression in colon tissues from AOM DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. B Representative immunoblot for AP2a and the loading control b-actin in AP2a OE and Mock cells. C Fold change in AP2a, Egfl7, and miR-126 expression in AP2a OE compared to Mock CMT93 cells as determined by qPCR (n = 3/group). D and E Fold change in miR-221, AP2a, and miR-126 expression in miR-221 OE (D) and miR-221 (E) silenced CMT93 compared to Mock cells (n = 3/group). F Fold change in miR-221 expression in colon tissues of AOM/DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. G Fold change in miR-221 expression in si-HB-EGF, si-EGFR, or si-HB-EGF/si-EGFR CMT93 cells treated with or without rec. HB-EGF compared to the gene expression in si-ctrl CMT93 by qPCR. H Cell proliferation of untreated si-Ctrl, si-miR- 221, and miR-221 OE cells as determined by cell counting (n = 6/group). Mean ± SEM, unpaired Student’s t test. *p < 0.05 and **p < 0.01.
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    Fig. 3 HB-EGF and EGFR control miR-221 expression that targets <t>AP2a</t> expression. A Fold change in AP2a expression in colon tissues from AOM DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. B Representative immunoblot for AP2a and the loading control b-actin in AP2a OE and Mock cells. C Fold change in AP2a, Egfl7, and miR-126 expression in AP2a OE compared to Mock CMT93 cells as determined by qPCR (n = 3/group). D and E Fold change in miR-221, AP2a, and miR-126 expression in miR-221 OE (D) and miR-221 (E) silenced CMT93 compared to Mock cells (n = 3/group). F Fold change in miR-221 expression in colon tissues of AOM/DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. G Fold change in miR-221 expression in si-HB-EGF, si-EGFR, or si-HB-EGF/si-EGFR CMT93 cells treated with or without rec. HB-EGF compared to the gene expression in si-ctrl CMT93 by qPCR. H Cell proliferation of untreated si-Ctrl, si-miR- 221, and miR-221 OE cells as determined by cell counting (n = 6/group). Mean ± SEM, unpaired Student’s t test. *p < 0.05 and **p < 0.01.
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    ap2a  (Developmental Studies Hybridoma Bank)
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    Fig. 3 HB-EGF and EGFR control miR-221 expression that targets <t>AP2a</t> expression. A Fold change in AP2a expression in colon tissues from AOM DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. B Representative immunoblot for AP2a and the loading control b-actin in AP2a OE and Mock cells. C Fold change in AP2a, Egfl7, and miR-126 expression in AP2a OE compared to Mock CMT93 cells as determined by qPCR (n = 3/group). D and E Fold change in miR-221, AP2a, and miR-126 expression in miR-221 OE (D) and miR-221 (E) silenced CMT93 compared to Mock cells (n = 3/group). F Fold change in miR-221 expression in colon tissues of AOM/DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. G Fold change in miR-221 expression in si-HB-EGF, si-EGFR, or si-HB-EGF/si-EGFR CMT93 cells treated with or without rec. HB-EGF compared to the gene expression in si-ctrl CMT93 by qPCR. H Cell proliferation of untreated si-Ctrl, si-miR- 221, and miR-221 OE cells as determined by cell counting (n = 6/group). Mean ± SEM, unpaired Student’s t test. *p < 0.05 and **p < 0.01.
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    Image Search Results


    Fig. 3 HB-EGF and EGFR control miR-221 expression that targets AP2a expression. A Fold change in AP2a expression in colon tissues from AOM DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. B Representative immunoblot for AP2a and the loading control b-actin in AP2a OE and Mock cells. C Fold change in AP2a, Egfl7, and miR-126 expression in AP2a OE compared to Mock CMT93 cells as determined by qPCR (n = 3/group). D and E Fold change in miR-221, AP2a, and miR-126 expression in miR-221 OE (D) and miR-221 (E) silenced CMT93 compared to Mock cells (n = 3/group). F Fold change in miR-221 expression in colon tissues of AOM/DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. G Fold change in miR-221 expression in si-HB-EGF, si-EGFR, or si-HB-EGF/si-EGFR CMT93 cells treated with or without rec. HB-EGF compared to the gene expression in si-ctrl CMT93 by qPCR. H Cell proliferation of untreated si-Ctrl, si-miR- 221, and miR-221 OE cells as determined by cell counting (n = 6/group). Mean ± SEM, unpaired Student’s t test. *p < 0.05 and **p < 0.01.

    Journal: Cell death & disease

    Article Title: Heparin-binding EGF-like growth factor via miR-126 controls tumor formation/growth and the proteolytic niche in murine models of colorectal and colitis-associated cancers.

    doi: 10.1038/s41419-024-07126-2

    Figure Lengend Snippet: Fig. 3 HB-EGF and EGFR control miR-221 expression that targets AP2a expression. A Fold change in AP2a expression in colon tissues from AOM DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. B Representative immunoblot for AP2a and the loading control b-actin in AP2a OE and Mock cells. C Fold change in AP2a, Egfl7, and miR-126 expression in AP2a OE compared to Mock CMT93 cells as determined by qPCR (n = 3/group). D and E Fold change in miR-221, AP2a, and miR-126 expression in miR-221 OE (D) and miR-221 (E) silenced CMT93 compared to Mock cells (n = 3/group). F Fold change in miR-221 expression in colon tissues of AOM/DSS mice compared to untreated control mice at day 84 (n = 3/group) by qPCR. G Fold change in miR-221 expression in si-HB-EGF, si-EGFR, or si-HB-EGF/si-EGFR CMT93 cells treated with or without rec. HB-EGF compared to the gene expression in si-ctrl CMT93 by qPCR. H Cell proliferation of untreated si-Ctrl, si-miR- 221, and miR-221 OE cells as determined by cell counting (n = 6/group). Mean ± SEM, unpaired Student’s t test. *p < 0.05 and **p < 0.01.

    Article Snippet: Cell lysates (2–50 μg proteins) were generated as described previously [6, 17] Membranes (Millipore, Immobilon) were probed with one of the following primary antibodies (1 μg/ml) overnight at 4 °C: C-terminal cytoplasmic domain of HB-EGF of mouse origin (Santa Cruz Biotech, sc1414), ADAM9 precursor (Santa Cruz Biotech, sc-377233), MMP7 (Santa Cruz Biotech, sc-515703), huADAM117 (Santa Cruz Biotech, sc-6416), p-ERK (Cell Signaling, #4370), CCL2 (Santa Cruz Biotech, sc-52701); AP2a (Cell Signaling, #3215); mouse b-actin (Cell Signaling, #4967).

    Techniques: Control, Expressing, Western Blot, Gene Expression, Cell Counting

    Fig. 4 Silencing of MMP7, ADAM9, and ADAM17 or MMPi treatment enhances miR-126 expression, while miR-126 restoration impairs their expression. A Fold change in ADAM17, miR-221, AP2a, or miR-126 expression in si-ADAM17, si-ADAM9, and si-MMP7 cells compared to expression in si-ctrl CMT93 cells (n = 3/group). B Representative immunoblots for MMP7, ADAM9, ADAM28 and b-actin in si-ctrl, si-MMP7, or si- ADAM9 and si-ADAM28 CMT93 cells. C Fold change in ADAM28, miR-221, AP2a, or miR-126 expression in si-ADAM28 CMT 93 cells (n = 3/group). D Fold change in miR-221, AP2a, and miR-126 expression in wild-type CMT93 cells treated with indicated concentrations of batimastat compared to expression in untreated ctr cells (n = 3/group). E Representative immunoblots for HB-EGF and b-actin in cell lysates from CRC cells treated without or with batimastat. F Fold change in HB-EGF, miR-221, miR-126, ADAM9, and CCL2 expression in Mock, miR-126 OE, and miR-126 KD cells treated with/without batimastat (n = 3/group). B-actin was run on a separate gel from the other proteins for all immunoblots. Mean ± SEM unpaired Student's t test. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Cell death & disease

    Article Title: Heparin-binding EGF-like growth factor via miR-126 controls tumor formation/growth and the proteolytic niche in murine models of colorectal and colitis-associated cancers.

    doi: 10.1038/s41419-024-07126-2

    Figure Lengend Snippet: Fig. 4 Silencing of MMP7, ADAM9, and ADAM17 or MMPi treatment enhances miR-126 expression, while miR-126 restoration impairs their expression. A Fold change in ADAM17, miR-221, AP2a, or miR-126 expression in si-ADAM17, si-ADAM9, and si-MMP7 cells compared to expression in si-ctrl CMT93 cells (n = 3/group). B Representative immunoblots for MMP7, ADAM9, ADAM28 and b-actin in si-ctrl, si-MMP7, or si- ADAM9 and si-ADAM28 CMT93 cells. C Fold change in ADAM28, miR-221, AP2a, or miR-126 expression in si-ADAM28 CMT 93 cells (n = 3/group). D Fold change in miR-221, AP2a, and miR-126 expression in wild-type CMT93 cells treated with indicated concentrations of batimastat compared to expression in untreated ctr cells (n = 3/group). E Representative immunoblots for HB-EGF and b-actin in cell lysates from CRC cells treated without or with batimastat. F Fold change in HB-EGF, miR-221, miR-126, ADAM9, and CCL2 expression in Mock, miR-126 OE, and miR-126 KD cells treated with/without batimastat (n = 3/group). B-actin was run on a separate gel from the other proteins for all immunoblots. Mean ± SEM unpaired Student's t test. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: Cell lysates (2–50 μg proteins) were generated as described previously [6, 17] Membranes (Millipore, Immobilon) were probed with one of the following primary antibodies (1 μg/ml) overnight at 4 °C: C-terminal cytoplasmic domain of HB-EGF of mouse origin (Santa Cruz Biotech, sc1414), ADAM9 precursor (Santa Cruz Biotech, sc-377233), MMP7 (Santa Cruz Biotech, sc-515703), huADAM117 (Santa Cruz Biotech, sc-6416), p-ERK (Cell Signaling, #4370), CCL2 (Santa Cruz Biotech, sc-52701); AP2a (Cell Signaling, #3215); mouse b-actin (Cell Signaling, #4967).

    Techniques: Expressing, Western Blot